VOLUME 44
, ISSUE 4ENG(Aug-2016,2016 ) >
List of Articles
Human Amniotic Membrane Transplantation - Different Modalities of its Use in Ophthalmology
Chintan Malhotra, Arun K Jain
Citation Information :
Malhotra C, Jain AK. Human Amniotic Membrane Transplantation - Different Modalities of its Use in Ophthalmology. 2016; 44 (4ENG):2-14.
The amniotic membrane (AM) is the inner layer of the fetal membranes and consists of 3 different layers: the epithelium, basement membrane and stroma which further consists of three contiguous but distinct layers: the inner compact layer, middle fibroblast layer and the outermost spongy layer. The AM has been shown to have anti-inflammatory, anti-fibrotic, anti-angiogenic as well as anti-microbial properties. Also because of its transparent structure, lack of immunogenicity and the ability to provide an excellent substrate for growth, migration and adhesion of epithelial corneal and conjunctival cells, it is being used increasingly for ocular surface reconstruction in a variety of ocular pathologies including corneal disorders associated with limbal stem cell deficiency, surgeries for conjunctival reconstruction, as a carrier for ex vivo expansion of limbal epithelial cells, glaucoma surgeries and sceral melts and perforations. However indiscriminate use of human AM needs to be discouraged as complications though infrequent can occur. These include risk of transmission of bacterial, viral or fungal infections to the recipient if the donors are not adequately screened for communicable diseases, if the membrane is not processed under sterile conditions or if storage is improper. Optimal outcomes can be achieved only with meticulous case selection. This review explores the ever-expanding ophthalmological indications for the use of human AM.
Davis JW. Skin transplantation with a review of 550 cases at the Johns Hopkins Hospital. Johns Hopkins Med J 1910; 15:307
de Roth A. Plastic repair of conjunctival defects with fetal membrane. Arch Ophthalmol 1940; 23: 522- 525
Batlle JF, Perdomo FJ. Placental membranes as a conjunctival substitute. Ophthalmol 1993: 100: A107
Fukuda K, Chikama T, Nakamura M, Nishida T. Differential distribution of sub-chains of the basement membrane components type IV collagen and laminin among the amniotic membrane, cornea and conunctiva. Cornea 1999; 18: 73-79
Baum J. Thygeson lecture. Amniotic membrane transplantation: why is it effective? Cornea 2002; 21: 339-341
Lee HS, Kim JC. Effect of amniotic fluid in corneal sensitivity and nerve regeneration after eximer laser ablation. Cornea 1996; 15: 517-524
Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol 2002; 86: 463-471
Meller D, Tseng SC. Conjunctival epithelial cell differentiation on amniotic membrane. Invest Ophthalmol Vis Sci 1999; 40: 878-886
Keene DR, Sakai LY, Lunstrum GP. Type VII collagen forms an extended network of anchoring fibrils. J Cell Biol 1987; 104: 611-621
Sonnenberg A, Calafat J, Janssen H, Daams H, van der Raaij-Helmer LM, Falcioni R, Kennel SJ, Aplin JD, Baker J, Loizidou M. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cellbasement membrane adhesion. J Cell Biol 1991; 113: 907-917
Terranova VP, Lyall RM. Chemotaxis of human gingival epithelial cells to laminin. A mechanism for epithelial cell apical migration. J Periodonto 1986; 57: 311-317
Guo M, Grinnell F. Basement membrane and human epidermal differentiation in vitro. J Invest Dermatol 1989; 93: 372-378
Kurpakus MA, Stock EL, Jones JC. The role of the basement membrane in differential expression of keratin proteins in epithelial cells. Dev Biol 1992; 150: 243-255
Streuli CH, Bailey N, Bissell MJ. Control of mammary epithelial differentiation: basement membrane induces tissuespecific gene expression in the absence of cell-cell interaction and morphological polarity. J Cell Biol 1991; 115: 1383-1395
Boudreau N, Sympson CJ, Werb Z, Bissell MJ. Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 1995; 267: 891-893
Boudreau N, Werb Z, Bissell MJ. Suppression of apoptosis by basement membrane requires three-dimensional tissue organization and withdrawal from the cell cycle. Proc Natl Acad Sci USA 1996; 93: 3509-3513
Lee SB, Li DQ, Tan DT, Meller DC, Tseng SC. Suppression of TGF-beta signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res 2000; 20: 325-334
Solomon A, Rosenblatt M, Monroy D, Ji Z, Pflugfelder SC, Tseng SC. Suppression of Interleukin 1 alpha and Interleukin 1 beta in the human limbal epithelial cells cultured on the amniotic membrane stromal matrix. Br J Ophthalmol 2001; 85: 444-449
Shimmura S, Shimazaki J, Ohashi Y, Tsubota K. Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea 2001; 20: 408-413
Hao Y, Ma DH, Hwang DG, Kim WS, Zhang F. Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea 2000; 19: 348-352
Rao TV, Chandrasekharam V. Use of dry human and bovine amnion as a biological dressing. Arch Surg 1981; 116: 891-896
Robson MC, Krizek TJ. The effect of human amniotic membranes on the bacteria population of infected rat burns. Ann Surg 1973; 177: 144-149
Kjaergaard N, Hein M, Hyttel L, Helmig RB, Schønheyder HC, Uldbjerg N, Madsen H. Antibacterial properties of human amnion and chorion in vitro. Eur J Obstet Gynecol Reprod Biol 2001; 94: 224-229
Kjaergaard N, Helmig RB, Schønheyder HC, Uldbjerg N, Hansen ES, Madsen H. Chorioamniotic membranes constitute a competent barrier to group b streptococcus in vitro. Eur J Obstet Gynecol Reprod Biol 1999; 83: 165-169
Gudson J. A Bactericidin for bacillus subtilis in pregnancy. J Immunol 1962; 88: 494-499
Galask RP, Snyder IS. Antimicrobial factors in amniotic fluid. Am J Obstet Gynecol 1970; 106: 59-65
Talmi YP, Sigler L, Inge E, Finkelstein Y, Zohar Y. Antibacterial properties of human amniotic membranes. Placenta 1991; 12: 285-288
Adinolfi M, Akle CA, McColl I, Fensom AH, Tansley L, Connolly P, Hsi BL, Faulk WP, Travers P, Bodmer WF. Expression of HLA antigens, beta 2-microglobulin and enzymes by human amniotic epithelial cells. Nature 1982; 295: 325-327 [PMID: 6173762 DOI: 10.1038/295325a0]
Akle CA, Adinolfi M, Welsh KI, Leibowitz S, McColl I. Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet 1981; 2: 1003-1005
Dua HS, Azuara-Blanco A. Amniotic membrane transplantation. Br J Ophthalmol 1999; 83: 748- 752
Kim JC, Tseng SC. Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea 1995; 14: 473-484
Kim JC, Tseng SC. The effects on inhibition of corneal neovascularization after human amniotic membrane transplantation in severely damaged rabbit corneas. Korean J Ophthalmol 1995; 9: 32-46
Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol 1997; 123: 303-312
Maral T, Borman H, Arslan H, Demirhan B, Akinbingol G, Haberal M. Effectiveness of human amnion preserved long-term in glycerol as a temporary biological dressing. Burns 1999; 25: 625-635
Adds PJ, Hunt CJ, Dart JK. Amniotic membrane grafts, “fresh” or frozen? A clinical and in vitro comparison. Br J Ophthalmol 2001; 85: 905-907
Kruse FE, Joussen AM, Rohrschneider K, You L, Sinn B, Baumann J, Völcker HE. Cryopreserved human amniotic membrane for ocular surface reconstruction. Graefes Arch Clin Exp Ophthalmol 2000; 238: 68-75
Kubo M, Sonoda Y, Muramatsu R, Usui M. Immunogenicity of human amniotic membrane in experimental xenotransplantation. Invest Ophthalmol Vis Sci 2001; 42: 1539-1546
Dua HS, Gomes JA, King AJ, Maharajan VS. The amniotic membrane in ophthalmology. Surv Ophthalmol 2004; 49: 51-77
Hu DJ, Basti S, Wen A, Bryar PJ. Prospective comparison of corneal re-epithelialization over the stromal and basement membrane surfaces of preserved human amniotic membrane. ARVO 2003; 3151
Seitz B, Resch MD, Schlötzer-Schrehardt U, Hofmann-Rummelt C, Sauer R, Kruse FE. Histopathology and ultrastructure of human corneas after amniotic membrane transplantation. Arch Ophthalmol 2006; 124: 1487-1490
Grueterich M, Tseng SC. Human limbal progenitor cells expanded on intact amniotic membrane ex vivo. Arch Ophthalmol 2002; 120: 783-790
Letko E, Stechschulte SU, Kenyon KR, Sadeq N, Romero TR, Samson CM, Nguyen QD, Harper SL, Primack JD, Azar DT, Gruterich M, Dohlman CH, Baltatzis S, Foster CS. Amniotic membrane inlay and overlay grafting for corneal epithelial defects and stromal ulcers. Arch Ophthalmol 2001; 119:659-663
Brown SI, Bloomfield SE, Tam W. The cornea-destroying enzyme of Pseudomonas aeruginosa. Invest Ophthalmol 1974; 13: 174-180
Buxton JN, Fox ML. Conjunctival flaps in the treatment of refractory pseudomonas corneal abscess. Ann Ophthalmol 1986; 18: 315-318
Hanada K, Shimazaki J, Shimmura S, Tsubota K. Multilayered amniotic membrane transplantation for severe ulceration of the cornea and sclera. Am J Ophthalmol 2001; 131: 324-331
Solomon A, Meller D, Prabhasawat P, John T, Espana EM, Steuhl KP, Tseng SC. Amniotic membrane grafts for nontraumatic corneal perforations, descemetoceles, and deep ulcers. Ophthalmology 2002; 109: 694-703
Jain AK, Sukhija J. Amniotic membrane transplantation in ocular rosacea. Ann Ophthalmol (Skokie) 2007; 39: 71-73
Kim HK, Park HS. Fibrin glue-assisted augmented amniotic membrane transplantation for the treatment of large noninfectious corneal perforations. Cornea 2009; 28: 170-176
Espana EM, Grueterich M, Sandoval H, Solomon A, Alfonso E, Karp CL, Fantes F, Tseng SC. Amniotic membrane transplantation for bullous keratopathy in eyes with poor visual potential. J Cataract Refract Surg 2003; 29: 279-284
Anderson DF, Prabhasawat P, Alfonso E, Tseng SC. Amniotic membrane transplantation after the primary surgical management of band keratopathy. Cornea 2001; 20: 354-361
Tseng SC, Prabhasawat P, Barton K, Gray T, Meller D. Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency. Arch Ophthalmol 1998; 116: 431-441
Prabhasawat P, Tseng SC. Impression cytology study of epithelial phenotype of ocular surface reconstructed by preserved human amniotic membrane. Arch Ophthalmol 1997; 115: 1360- 1367
Prabhasawat P, Barton K, Burkett G, Tseng SC. Comparison of conjunctival autografts, amniotic membrane grafts, and primary closure for pterygium excision. Ophthalmology 1997; 104: 974- 985
Solomon A, Pires RTF, Tseng SCG: Amniotic membrane transplantation after extensive removal of primary and recurrent pterygia. Ophthalmol 2001; 108: 449-460
Ma DH, See LC, Liau SB, Tsai RJ. Amniotic membrane graft for primary pterygium: comparison with conjunctival autograft and topical mitomycin C treatment. Br J Ophthalmol 2000; 84: 973- 978
Jain AK, Bansal R, Sukhija J. Human amniotic membrane transplantation with fibrin glue in management of primary pterygia: a new tuck-in technique. Cornea 2008; 27: 94-99
Paridaens D, Beekhuis H, van Den Bosch W, Remeyer L, Melles G. Amniotic membrane transplantation in the management of conjunctival malignant melanoma and primary acquired melanosis with atypia. Br J Ophthalmol 2001; 85: 658-661
John T. Human amniotic membrane transplantation: past, present, and future. Ophthalmol Clin North Am 2003; 16: 43-65; vi
Rahman I, Said DG, Maharajan VS, Dua HS. Amniotic membrane in ophthalmology: indications and limitations. Eye (Lond) 2009; 23: 1954-1961
Maharajan VS, Shanmuganathan V, Currie A, Hopkinson A, Powell-Richards A, Dua HS. Amniotic membrane transplantation for ocular surface reconstruction: indications and outcomes. Clin Experiment Ophthalmol 2007; 35: 140-147
Lavker RM, Tseng SC, Sun TT. Corneal epithelial stem cells at the limbus: looking at some old problems from a new angle. Exp Eye Res 2004; 78: 433-446
Schwab IR, Reyes M, Isseroff RR. Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 2000; 19: 421-426
Tsai RJ, Li LM, Chen JK. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med 2000; 343: 86-93
Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 2001; 108: 1569-1574
Fujishima H, Shimazaki J, Shinozaki N, Tsubota K. Trabeculectomy with the use of amniotic membrane for uncontrollable glaucoma. Ophthalmic Surg Lasers 1998; 29: 428-431
Nagai-Kusuhara A, Nakamura M, Fujioka M, Negi A. Longterm results of amniotic membrane transplantation-assisted bleb revision for leaking blebs. Graefes Arch Clin Exp Ophthalmol 2008; 246: 567-571
Budenz DL, Barton K, Tseng SC. Amniotic membrane transplantation for repair of leaking glaucoma filtering blebs. Am J Ophthalmol 2000; 130: 580-588
Oh JH, Kim JC. Repair of scleromalacia using preserved scleral graft with amniotic membrane transplantation. Cornea 2003; 22: 288-293
Ma DH, Wang SF, Su WY, Tsai RJ. Amniotic membrane graft for the management of scleral melting and corneal perforation in recalcitrant infectious scleral and corneoscleral ulcers. Cornea 2002; 21: 275-283
Tay E, Utine CA, Akpek EK. Crescenteric amniotic membrane grafting in keratoprosthesisassociated corneal melt. Arch Ophthalmol 2010; 128: 779-782 [PMID: 20547957 DOI: 10.1001/archophthalmol.2010.95]
Sridhar MS, Sangwan VS, Bansal AK, Rao GN. Amniotic membrane transplantation in the management of shield ulcers of vernal keratoconjunctivitis. Ophthalmology 2001; 108: 1218- 1222
Murube J, Olivares C, Murube E: Treatment of dry eye by punctum patch. Orbit 1995; 14: 1-7 [DOI: 10.3109/016768395 09149156]
Ti SE, Tow SL, Chee SP. Amniotic membrane transplantation in entropion surgery. Ophthalmology 2001; 108: 1209-1217 [PMID: 11425677
Marangon FB, Alfonso EC, Miller D, Remonda NM, Muallem MS, Tseng SC. Incidence of microbial infection after amniotic membrane transplantation. Cornea 2004; 23: 264-269
Khokhar S, Sharma N, Kumar H, Soni A. Infection after use of nonpreserved human amniotic membrane for the reconstruction of the ocular surface. Cornea 2001; 20: 773-774